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bigdye terminator chemistry  (Thermo Fisher)


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    Thermo Fisher bigdye terminator chemistry
    Bigdye Terminator Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bigdye+terminator+chemistry/bigdye+terminator+v3+1+cycle+sequencing+kit/pmc12211047-52-12-15
    Average 90 stars, based on 1 article reviews
    bigdye terminator chemistry - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Alu-Mediated Duplication and Deletion of Exon 11 Are Frequent Mechanisms of PALB2 Inactivation, Predisposing Individuals to Hereditary Breast-Ovarian Cancer Syndrome.
    Article Snippet: Long-range PCRs were performed using the Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and PCR products were sequenced by Sanger sequencing. .. Briefly, PCR products were sequenced on the SeqStudio Genetic Analyzer sequencer (Applied Biosystems, Thermo Fisher, Monza, Italy) using BigDye Terminator Chemistry (Applied Biosystems, Thermo Fisher Scientific, Monza, Italy) according to the manufacturers’ recommendations. .. The results were analyzed with the SeqScanner 2 v2.0 and SeqScape v2.6 software (Applied Biosystems, Thermo Fisher Scientific, Monza, Italy).

    Article Title: A Countrywide Survey of hrp2/3 Deletions and kelch13 Mutations Co-occurrence in Ethiopia
    Article Snippet: PCR products were run on 2% agarose gel with a 1-kb ladder (New England Biolabs), and the bands at 849 base pairs were purified using the QI Quick Gel Extraction Kit (Qiagen). .. Bidirectional Sanger sequencing of the purified PCR products was performed using BigDye Terminator chemistry with an Applied Biosystems 3730XL instrument at the Centre for Health Genomics and Informatics (University of Calgary). .. Sequencing results were assessed individually by analyzing both strands with an MAFFT local pairwise alignment tool on a Benchling cloud-based platform (MAFFT version 7) against the P. falciparum 3D7 k13 reference sequence (PlasmoDB: PF3D7_1343700). shows the primer sequences used for nested PCR.

    Article Title: Expansion of the Genotypic and Phenotypic Spectrum of TCTN3 -Related Joubert Syndrome.
    Article Snippet: .. The sequencing of PCR products was performed using BigDye Terminator chemistry (Thermo Fisher Scientific, Vilnius, Lithuania) in both forward and reverse directions on an ABI 3130 automated sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Amplification:

    Article Title: An amplicon-based approach for full-genome characterization of HPV16
    Article Snippet: .. Briefly, 2 μL of amplified DNA was subjected to bidirectional sequencing with BigDye Terminator Chemistry (Thermo Fisher Scientific) with an analysis of the reaction products on an ABI sequencer. ..

    Sequencing:

    Article Title: An amplicon-based approach for full-genome characterization of HPV16
    Article Snippet: .. Briefly, 2 μL of amplified DNA was subjected to bidirectional sequencing with BigDye Terminator Chemistry (Thermo Fisher Scientific) with an analysis of the reaction products on an ABI sequencer. ..

    Article Title: A Countrywide Survey of hrp2/3 Deletions and kelch13 Mutations Co-occurrence in Ethiopia
    Article Snippet: PCR products were run on 2% agarose gel with a 1-kb ladder (New England Biolabs), and the bands at 849 base pairs were purified using the QI Quick Gel Extraction Kit (Qiagen). .. Bidirectional Sanger sequencing of the purified PCR products was performed using BigDye Terminator chemistry with an Applied Biosystems 3730XL instrument at the Centre for Health Genomics and Informatics (University of Calgary). .. Sequencing results were assessed individually by analyzing both strands with an MAFFT local pairwise alignment tool on a Benchling cloud-based platform (MAFFT version 7) against the P. falciparum 3D7 k13 reference sequence (PlasmoDB: PF3D7_1343700). shows the primer sequences used for nested PCR.

    Article Title: Expansion of the Genotypic and Phenotypic Spectrum of TCTN3 -Related Joubert Syndrome.
    Article Snippet: .. The sequencing of PCR products was performed using BigDye Terminator chemistry (Thermo Fisher Scientific, Vilnius, Lithuania) in both forward and reverse directions on an ABI 3130 automated sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Purification:

    Article Title: A Countrywide Survey of hrp2/3 Deletions and kelch13 Mutations Co-occurrence in Ethiopia
    Article Snippet: PCR products were run on 2% agarose gel with a 1-kb ladder (New England Biolabs), and the bands at 849 base pairs were purified using the QI Quick Gel Extraction Kit (Qiagen). .. Bidirectional Sanger sequencing of the purified PCR products was performed using BigDye Terminator chemistry with an Applied Biosystems 3730XL instrument at the Centre for Health Genomics and Informatics (University of Calgary). .. Sequencing results were assessed individually by analyzing both strands with an MAFFT local pairwise alignment tool on a Benchling cloud-based platform (MAFFT version 7) against the P. falciparum 3D7 k13 reference sequence (PlasmoDB: PF3D7_1343700). shows the primer sequences used for nested PCR.

    Electrophoresis:

    Article Title: Asymptomatic carriage of intestinal protists is common in children in Lusaka Province, Zambia
    Article Snippet: A 100-bp DNA ladder (Boehringer Mannheim GmbH, Baden-Wurttemberg, Germany) was used for the sizing of obtained amplicons. .. Positive-PCR products were sequenced in both directions using internal primer sets ( ) by capillary electrophoresis using the BigDye Terminator chemistry (Applied Biosystems) on an on ABI PRISM 3130 automated DNA sequencer. ..

    Article Title: Asymptomatic carriage of intestinal protists is common in children in Lusaka Province, Zambia.
    Article Snippet: A 100-bp DNA ladder (Boehringer Mannheim GmbH, Baden-Wurttemberg, Germany) was used for the sizing of obtained amplicons. .. Positive-PCR products were sequenced in both directions using internal primer sets (S1 Table) by capillary electrophoresis using the BigDye Terminator chemistry (Applied Biosystems) on an on ABI PRISM 3130 automated DNA sequencer. ..

    Article Title: Free-Ranging Wolves (Canis lupus) are Natural Reservoirs of Intestinal Microeukaryotes of Public Health Significance in Southwestern Europe.
    Article Snippet: Introduction: Intestinal microeukaryote parasites are major contributors to the burden of diarrhoea in humans and domestic animals, but their epidemiology in wildlife is not fully understood.. We investigated the frequency, genetic diversity and zoonotic potential of protists of animal and public health significance in freeranging grey wolf (Canis lupus) populations in southwestern Europe.. Methods: Individually formed faecal samples collected from necropsied wolves or scat trails in Italy (n = 47), Portugal (n = 43) and Spain (n = 225) during the period 2011–2023 were retrospectively analysed using molecular (PCR and Sanger sequencing) methods.

    DNA Sequencing:

    Article Title: Free-Ranging Wolves (Canis lupus) are Natural Reservoirs of Intestinal Microeukaryotes of Public Health Significance in Southwestern Europe.
    Article Snippet: Introduction: Intestinal microeukaryote parasites are major contributors to the burden of diarrhoea in humans and domestic animals, but their epidemiology in wildlife is not fully understood.. We investigated the frequency, genetic diversity and zoonotic potential of protists of animal and public health significance in freeranging grey wolf (Canis lupus) populations in southwestern Europe.. Methods: Individually formed faecal samples collected from necropsied wolves or scat trails in Italy (n = 47), Portugal (n = 43) and Spain (n = 225) during the period 2011–2023 were retrospectively analysed using molecular (PCR and Sanger sequencing) methods.



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